SCIENCE
oligoDOM® technology platform uniquely combines two well-established approaches to trigger powerful immune responses and revolutionize next generation vaccines
- A virus-like-particle (VLP) approach making the antigen much more visible for the immune system
- A positively charged tail facilitating entry in dendritic cells to stimulate CD8 T-cell responses
oligoDOM® (OVX313) is derived from our first generation technology (IMX313). The technology has been improved with the addition of a highly positively charged polyarginine tail to facilitate entry in dendritic cells and elicit much higher CD8 T-cell responses
oligoDOM® (OVX313) technology platform:
- is a versatile technology that can be used with a full antigen or a long peptide
- triggers robust cellular and antibody immune responses, with unprecedented cytotoxic T-cell activation (CD8)
- can be used in various vaccine technologies (recombinant proteins, RNA and DNA vaccines, viral vectors...) as it can be encoded as a DNA or RNA sequence
- relies on more than 12 years of R&D and is protected by two patent families
- is currently in clinical development in two indications (Malaria (first generation) and Flu)
When associated to an antigen, oligoDOM® forms a large and positively charged recombinant protein that activates the 3 arms of immunity with unprecedented CD8 T-cell responses
REFERENCES
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Universal Influenza Vaccine Program
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OVX836 a recombinant nucleoprotein vaccine inducing cellular responses and protective efficacy against multiple influenza A subtypes. Del Campo J, Pizzorno A, Djebali S, Bouley J, Haller M, Pérez-Vargas J, Lina B, Boivin G, Hamelin M-E, Nicolas F, Le Vert A, Leverrier Y, Rosa-Calatrava M, Marvel J, Hill F. 2019 Jan. doi: 10.1038/s41541-019-0098-4.
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oligoDOM® (OVX313) – Second generation technology platform with a positively charge core
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Minor capsid protein L2 polytope induces broad protection against oncogenic and mucosal human papillomaviruses. Pouyanfard S, Spagnoli G, Bulli L, Balz K, Yang F, Odenwald C, Seitz H, Mariz FC, Bolchi A, Ottonello S, Müller M. 2018 Feb. J Virol 92:e01930-17. doi: 10.1128/JVI.01930-17.
Broadly neutralizing antiviral responses induced by a single-molecule HPV vaccine based on thermostable thioredoxin-L2 multiepitope nanoparticles. Spagnoli G, Pouyanfard S, Cavazzini D, Canali E, Maggi S, Tommasino M, Bolchi A, Müller M, Ottonello S. Sci Rep. 2017; 7: 18000. Published online 2017 Dec 21. doi: 10.1038/s41598-017-18177-1.
Broadly neutralizing antiviral responses induced by a single-molecule HPV vaccine based on thermostable thioredoxin-L2 multiepitope nanoparticles. Spagnoli G, Pouyanfard S, Cavazzini D, Canali E, Maggi S, Tommasino M, Bolchi A, Müller M, Ottonello S. Sci Rep. 2017; 7: 18000. Published online 2017 Dec 21. doi: 10.1038/s41598-017-18177-1.
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IMX313 – First generation technology platform
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SnoopLigase peptide-peptide conjugation enables modular vaccine assembly. Andersson AC, Buldun CM, Pattinson DJ, Draper SJ, Howarth M.Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.1038/s41598-019-40985-w.
Combination of RTS,S and Pfs25-IMX313 Induces a Functional Antibody Response Against Malaria Infection and Transmission in Mice. Brod F, Miura K, Taylor I, Li Y, Marini A, Salman AM, Spencer AJ, Long CA, Biswas S.Front Immunol. 2018 Dec 4;9:2780. doi: 10.3389/fimmu.2018.02780.
Dual Plug-and-Display Synthetic Assembly Using Orthogonal Reactive Proteins for Twin Antigen Immunization. Brune KD, Buldun CM, Li Y, Taylor IJ, Brod F, Biswas S, Howarth M.Bioconjug Chem. 2017 May 17;28(5):1544-1551. doi: 10.1021/acs.bioconjchem.7b00174.
A HIV-Tat/C4-binding protein chimera encoded by a DNA vaccine is highly immunogenic and contains acute EcoHIV infection in mice. Tomusange K, Wijesundara D, Gummow J, Garrod T, Li Y, Gray L, Churchill M, Grubor-Bauk B, Gowans EJ.Sci Rep. 2016 Jun 30;6:29131. doi: 10.1038/srep29131.
Enhancing immunogenicity and transmission-blocking activity of malaria vaccines by fusing Pfs25 to IMX313 multimerization technology. Li Y, Leneghan DB, Miura K, Brian IJ, Dicks MD, Fyfe AJ, Zakutansky SE, De Cassan S, Long CA, Draper SJ, Hill AV, Hill F, Biswas S. Sci Rep. 2016 January 8;6:18848. doi: 10.1038/srep18848.
T Cell Responses Induced by Adenoviral Vectored Vaccines Can Be Adjuvanted by Fusion of Antigen to the Oligomerization Domain of C4b-Binding Protein. Forbes EK, de Cassan SC, Llewellyn D, Biswas S, Goodman AL, Cottingham MG, Long CA, Pleass RJ, Hill AV, Hill F, Simon J. Draper. PLoS ONE. 2012 September;7(9):e44943. doi:10.1371/journal.pone.0044943.
Fusion of the Mycobacterium tuberculosis antigen 85A to an oligomerization domain enhances its immunogenicity in both mice and non-human primates. Spencer AJ, Hill F, Honeycutt JD, Cottingham MG, Bregu M, Rollier CS, Furze J, Draper SJ, Søgaard KC, Gilbert SC, Wyllie DH, Hill AVS. PLoS ONE. 2012 March;7(3): e33555. doi:10.1371/journal.pone.0033555.
Immunocontraception in male feral swine treated with a recombinant gonadotropin-releasing hormone vaccine. Campbell TA, Garcia MR, Miller LA, Ramirez MA, Long DB, Marchand JB, Hill F. JSHAP. May 2010; 18(3):118-124.
The oligomerization domain of C4-binding protein (C4bp) acts as an adjuvant, and the fusion protein comprised of the 19-kilodalton merozoite surface protein 1 fused with the murine C4bp domain protects mice against malaria. Ogun SA, Dumon-Seignovert L, Marchand JB, Holder AA, Hill F. Infect Immun. 2008 Aug;76(8):3817-3823. doi:10.1128/IAI.01369-07.
Effective induction of high-titer antibodies by viral vector vaccines. Draper SJ, Moore AC, Goodman AL, Long CA, Holder AA, Gilbert SC, Hill F, Hill AV. Nat Med. 2008 Aug;14(8):819-821. doi: 10.1038/nm.1850.
Combination of RTS,S and Pfs25-IMX313 Induces a Functional Antibody Response Against Malaria Infection and Transmission in Mice. Brod F, Miura K, Taylor I, Li Y, Marini A, Salman AM, Spencer AJ, Long CA, Biswas S.Front Immunol. 2018 Dec 4;9:2780. doi: 10.3389/fimmu.2018.02780.
Dual Plug-and-Display Synthetic Assembly Using Orthogonal Reactive Proteins for Twin Antigen Immunization. Brune KD, Buldun CM, Li Y, Taylor IJ, Brod F, Biswas S, Howarth M.Bioconjug Chem. 2017 May 17;28(5):1544-1551. doi: 10.1021/acs.bioconjchem.7b00174.
A HIV-Tat/C4-binding protein chimera encoded by a DNA vaccine is highly immunogenic and contains acute EcoHIV infection in mice. Tomusange K, Wijesundara D, Gummow J, Garrod T, Li Y, Gray L, Churchill M, Grubor-Bauk B, Gowans EJ.Sci Rep. 2016 Jun 30;6:29131. doi: 10.1038/srep29131.
Enhancing immunogenicity and transmission-blocking activity of malaria vaccines by fusing Pfs25 to IMX313 multimerization technology. Li Y, Leneghan DB, Miura K, Brian IJ, Dicks MD, Fyfe AJ, Zakutansky SE, De Cassan S, Long CA, Draper SJ, Hill AV, Hill F, Biswas S. Sci Rep. 2016 January 8;6:18848. doi: 10.1038/srep18848.
T Cell Responses Induced by Adenoviral Vectored Vaccines Can Be Adjuvanted by Fusion of Antigen to the Oligomerization Domain of C4b-Binding Protein. Forbes EK, de Cassan SC, Llewellyn D, Biswas S, Goodman AL, Cottingham MG, Long CA, Pleass RJ, Hill AV, Hill F, Simon J. Draper. PLoS ONE. 2012 September;7(9):e44943. doi:10.1371/journal.pone.0044943.
Fusion of the Mycobacterium tuberculosis antigen 85A to an oligomerization domain enhances its immunogenicity in both mice and non-human primates. Spencer AJ, Hill F, Honeycutt JD, Cottingham MG, Bregu M, Rollier CS, Furze J, Draper SJ, Søgaard KC, Gilbert SC, Wyllie DH, Hill AVS. PLoS ONE. 2012 March;7(3): e33555. doi:10.1371/journal.pone.0033555.
Immunocontraception in male feral swine treated with a recombinant gonadotropin-releasing hormone vaccine. Campbell TA, Garcia MR, Miller LA, Ramirez MA, Long DB, Marchand JB, Hill F. JSHAP. May 2010; 18(3):118-124.
The oligomerization domain of C4-binding protein (C4bp) acts as an adjuvant, and the fusion protein comprised of the 19-kilodalton merozoite surface protein 1 fused with the murine C4bp domain protects mice against malaria. Ogun SA, Dumon-Seignovert L, Marchand JB, Holder AA, Hill F. Infect Immun. 2008 Aug;76(8):3817-3823. doi:10.1128/IAI.01369-07.
Effective induction of high-titer antibodies by viral vector vaccines. Draper SJ, Moore AC, Goodman AL, Long CA, Holder AA, Gilbert SC, Hill F, Hill AV. Nat Med. 2008 Aug;14(8):819-821. doi: 10.1038/nm.1850.